Nitrate is the main nitrogen (N) source for cereal crops such as wheat, thus understanding nitrate signaling in cereal crops is important and valuable for engineering crops with improved N use efficiency. Although several regulators have been identified in nitrate sensing and signaling in Arabidopsis, the equivalent information in cereals is missing. Specific nitrate transporters were found to regulate seed nitrate content and lateral root formation in Arabidopsis. A nitrate-inducible NAC transcription factor in wheat that is involved in regulating grain nitrate content and lateral root formation has recently been identified. This research will identify if wheat also has these nitrate-sensitive processes regulated by specific transporters.